Streck Cell‑Free DNA BCT, EDTA, and Acid Citrate Dextrose (ACD) tubes. Manual sample processing 8 individual serum samples can be processed in less than an hour.Ĭell‑Free DNA Isolation Kit is optimized for samples collected in.KingFisher ™ Duo Prime Magnetic Particle Processor (6‑well deep‑well setting).Three optimized methods are included:įlex Magnetic Particle Processor with 24 Deep Well Head (24DW This guide describes isolation of cfDNA from plasma and serum samples. SILANE technology and extraction chemistry, ensuring reproducible recovery of high‑quality cell‑free DNA (cfDNA) that is suitable for a broad range of applications, including sequencing, genotyping, and qPCR. The kit usesĬell‑Free DNA Isolation Kit is designed for The Applied Biosystems isolation of circulating DNA from cell‑free human plasma and serum. IMPORTANT! Before using this product, read and understand the information in theĦ Product information Product description MagMAX ™ Cell ‑Free DNA Isolation Kit User Guide About this guide Revision history 26ĪPPENDIX C Yield and quality measurement. Lyse the plasma samples and bind the cfDNA to the beads. MagMAX ™ Cell ‑Free DNA Isolation Kit User GuideĪPPENDIX B Alternate protocol for isolation of higher concentration cfDNA. Option 2: Lyse the plasma samples (without PK) and bind the cfDNA to the beads. Option 1: Lyse the plasma samples (with PK) and bind the cfDNA to the beads. (Optional) Treat the samples with Proteinase K. TaqMan is a trademark of Roche Molecular Systems, Inc., used under permission and license. Agilent is a trademark of Agilent Technologies, Inc. Eppendorf and ThermoMixer are trademarks of Eppendorf AG. By use of this product, you accept the terms and conditions of all applicableĪll trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. This product may be covered by one or more Limited Use Label Licenses.

PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT. TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, The information in this guide is subject to change without notice.

MagMAX ™ Cell ‑Free DNA Isolation Kit Isolation of cfDNA from plasma and serum samplesįor Research Use Only.

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